CV Alice-Corina Ceacareanu

Alice-Corina Ceacareanu

8315 Trinity Road

Memphis, TN 38018

Cell: 901-428-6840   Home & Fax: 901-309-9950

alice.ceacareanu@bmhcc.org

 


Education

 

Ø       Doctor of Pharmacy. University of Tennessee, College of Pharmacy. Memphis. 2007

Ø       Bachelor of Pharmacy. University of Medicine and Pharmacy "Carol Davila", College of Pharmacy. Bucharest, Romania. 2001

Ø       Doctor of Philosophy, Biology - Molecular Genetics. University of Bucharest. 2001

Ø       Bachelor of Science, Biochemistry. University of Bucharest. 1997

Fellowships

 

University of Tennessee, College of Medicine; Memphis, TN                2002 - 2005

      Cardiovascular Disease Research

 

University of Tennessee, College of Medicine; Memphis, TN                2001 - 2002

      Pharmacology Research

 

Institute of Human Biology; Hamburg, Germany                                    2000

      Pre-Doctoral Research in Genomic Polymorphism

 

Max-Planck Institute for Molecular Physiology; Dortmund, Germany     1999 - 2000

      Pre-Doctoral Research in Gene Cloning and Molecular Transport

Professional Experience

 

State University of New York, Buffalo, NY                                             2008 July -

School of Pharmacy and Pharmaceutical Sciences

      Assistant Professor of Pharmacy Practice - Oncology

 

Baptist Memorial Hospital; Memphis, TN                                              2007 - 2008

      Pharmacy Practice Residency

 

University of Tennessee, College of Pharmacy; Memphis, TN              2007

      Instructor of Clinical Pharmacy

 

Blue Cross Blue Shield of Louisiana; Baton Rouge, LA                         2006

      Managed Care Pharmacy Intern, Oncology

Ø       10-week Pfizer/AMCP co-sponsored internship

 

University of Tennessee, College of Medicine; Memphis, TN                2004 - 2005

      Physiology Dept. Research Associate

 

University of Bucharest; Bucharest, Romania                                        1998 - 1999

      Assistant Professor of Human Genetics

 

Cantacuzino Institute, Bucharest, Romania                                           

Ø       Biochemistry Specialist                                                              1997 - 1998

Ø       Research Assistant                                                                    1996 - 1997

Ø       Volunteer Research Assistant                                                     1994 - 1996

Professional Memberships

 

Ø       American College of Clinical Pharmacy (ACCP), Member

Ø       American Society of Health-System Pharmacists (ASHP), Member

Ø       Editorial Advisory Board of the Journal of Managed Care Pharmacy, Reviewer

Ø       Tennessee Pharmacist Association (TPA), Member

Ø       Academy of Managed Care Pharmacy (AMCP), Member

Ø       White Book of Romanian Scientific Community, www.ad-astra.ro/aceacareanu, Lifetime member

Ø       American Pharmacist Association - ASP, Member

Ø       Who's Who Empire of Executives and Professionals, Lifetime Member

Ø       American Heart Association (AHA), Professional Member

Ø       American Association for Advancement in Science (AAAS), Member

Ø       New York Academy of Sciences (NYAS), Professional Member

Ø       International Union of Immunology Societies (IUIS), Professional Member

Ø       Federation of European Biochemistry Societies (FEBS), Professional Member

 

Licensure

 

Ø       Registered Pharmacist in Tennessee

Ø       Registered Pharmacist in Mississippi

Ø       Registered Pharmacist in Pennsylvania

Ø       Registered Pharmacist in North Carolina

Ø       Registration as a Pharmacist in New York complete. Approved to take law and practical test

Ø       Registration as a Pharmacist in California complete. Approved to take CPJE

Ø       Registration as a Pharmacist in Texas complete. Approved to take MPJE

Ø       Registration as a Pharmacist in Virginia complete. Approved to take MPJE

Ø       Registration as a Pharmacist in Massachusetts complete. Approved to take law test

 

Certifications

 

Ø       Advanced Cardiac Life Support (2007)

Ø       Basic Life Support (2007)

 

Supervising Experience

 

 

Principal Investigator:

Ø       Generating protocol-based preprinted order form for Rituximab-Ifosfamide-Carboplatinum-Etoposide (RICE) chemo-regimen in BMHCC-Memphis setting (P&T Committee 2007)

Ø       Iron Dextran test dose administration safety policy in hospital setting (P&T Committee 2007)

Ø       Guidelines of prescribing and providing specialty products at BMHCC - Memphis (P&T Committee 2007)

Ø       Evidence-based pharmacokinetics guidance in vancomycin dosing (P&T Committee 2007)

Ø       An evaluation of the appropriateness of antiemetic premedication in oncology patients receiving chemotherapy (2007)

Ø       Analysis of inappropriate use of oral chemotherapy in Louisiana (2006)

Ø       Counter-regulatory function of PTP- PEST on growth factor-induced activation of Rac1 GTP-ase (2002-2004)

Ø       Nitric oxide effect on growth factor-induced smooth muscle cell (SMC) motility is mediated by PTP-PEST/p130cas (2003-2005)

Ø       Effect of chronic insulin on the inhibitory role of nitric oxide in regulation of motility of aortic smooth muscle cells (2002-2005)

Ø       Rac1-mediated H2O2 production and regulation of catalase levels in primary aortic smooth muscle cells (2002-2003)

Ø       Interaction between type V adenylyl cyclase and b2-adrenergic receptor in intact human embryonic kidney cells (2001-2002)

 

Co-Investigator:

Ø       Counter-regulatory function of PTP 1B growth factor-induced motility and proliferation of cultured SMC in neointima formation (2003-2005)

Ø       The role of a protein tyrosine phosphatase, PTP1B, as mediator of nitric oxide (NO)-induced inhibition of aortic smooth muscle cell motility and proliferation in culture, and neointima formation in vivo (2003-2005)

Ø       Regulation of PDGF Receptor activation and downstream signaling controls activation of Rac1 and PDGF-induced SMC motility

Ø       Nitric oxide-induced regulation of PTP-PEST activity and p130cas phosphorylation (2001-2003)

Ø       Protein kinase G role in NO-induced inhibition of SMC motility (2001-2003)

Ø       Variation of H2O2 levels in growth factor stimulated aortic smooth muscle cells (2001-2003)

Ø       Role of CRK in NO-induced inhibition in aortic smooth muscle cells motility (2001-2002)

Ø       Research experience coordinator for undergraduate students while working for UTHSC - (2001-2005)

 

Presented and Published Papers

 

2007

Ø       A Selective Review of Beta-blockers use in Patients undergoing CABG Surgery - Baptist Memorial Hospital, Memphis, TN, November 2nd, 2007

Ø       Standard Therapy in Chronic Heart Failure Management - Baptist Memorial Hospital, Memphis, TN, October 5th, 2007

Ø       Current Guidelines in Pain Management - Baptist Memorial Hospital, Memphis, TN, September 25th - 27th, 2007

 

2006

Ø       "Analysis of inappropriate use of oral chemotherapy in a Southern health plan" (Poster). Alice-Corina Ceacareanu, Milam Ford, Imelda Coleman, Mollie Cannon. Academy of Managed Care Pharmacy Educational Conference, Chicago, October 4-7 2006

Ø       "Nitric oxide attenuates IGF-I-induced aortic smooth muscle cell motility by decreasing Rac1 activity: essential role of PTP-PEST and p130cas". Ceacareanu Alice-Corina, Ceacareanu Bogdan, Zhuang Daming, Chang Yingzi, Ray Ramesh M, Desai Leena, Chapmann Kenneth E, Waters Christopher M, Hassid Aviv. Am J Physiol Cell Physiol. 2006 Apr;290(4):C1263-70

Ø       "Counter-regulatory function of protein tyrosine phosphatase 1B in platelet-derived growth factor- or fibroblast growth factor-induced motility and proliferation of cultured smooth muscle cells and in neointima formation".Chang Y, Ceacareanu B, Zhuang D, Zhang C, Pu Q, Ceacareanu AC, Hassid A. Arterioscler Thromb Vasc Biol. 2006 Mar;26(3):501-7

 

2005

Ø       "Essential role of protein kinase G and decreased cytoplasmic Ca2+ levels in NO-induced inhibition of rat aortic smooth muscle cell motility".Zhuang D, Ceacareanu AC, Ceacareanu B, Hassid A. Am J Physiol Heart Circ Physiol. 2005 Apr;288(4):H1859-66.

Ø       "Rac1 induces up-regulation of PTP-mRNA levels in cultured rat aortic SMC" (Poster). Alice-Corina Ceacareanu, Yingzi Chang, Bogdan Ceacareanu, Daming Zhuang, Aviv Hassid. Poster session -XIII th FASEB Meeting, San Diego, Apr 2005

Ø       "Role of small GTPase Rac1 in redox regulation of aortic smooth muscle cells". Alice-Corina Ceacareanu. Invited presentation for the seminar of the Physiology Department, UTHSC - Jan, 6th, 2005

 

2004

Ø       "Nitric oxide attenuates IGF-1-induced aortic smooth muscle cell motility by decreasing Rac1 activity: essential role of PTP-PEST and p130cas" (Poster). Ceacareanu AC, Ceacareanu B, Zhuang D, Chang Y, Ray RM, Desai L, Chapman KE, Waters CM, Hassid A. Circulation vol 110, No 17, October 26, 2004 - American Heart Association Meeting (New Orleans 2004)

Ø       "Nitric oxide attenuates insulin- or IGF1-stimulated aortic smooth muscle cell motility by hydrogen peroxide: Essential role of cyclic GMP". Daming Zhuang, Alice-Corina Ceacareanu, Yi Lin, Bogdan Ceacareanu, Madhulika Dixit2, Kenneth Chapman, Christopher M. Waters, Gadiparthi N. Rao and Aviv Hassid. Am J Physiol Heart Circ Physiol. 2004 Jun;286(6):H2103-12

Ø       "Distribution of D1S80 alleles and their Tsp509I subtypes in Romanian population". Alice-Corina Ceacareanu, Bogdan Ceacareanu, Augustin Ofiteru, Alexander Rodewald, Geza Szegli. Journal of Forensic Sciences 2004 Jul;49(4):865

 

2003

Ø       "NO induced inhibition of aortic smooth muscle cell motility: role of protein tyrosine phosphatase-PEST and adapter proteins p130cas and CRK ". Yi Lin, Alice-Corina Ceacareanu, Aviv Hassid. Am J Physiol Heart Circ Physiol. 2003 Aug;285(2):H710-21

 

2002

Ø       "Interaction of yeast Rab geranylgernyl transferase with its protein and lipid substrates". B Dursina, NH Thomä, V Sidorovitch, A Niculae, A Iakovenko, A Rak, S Albert, AC Ceacareanu, C Herrmann, RS Goody, K Allexandrov. Biochemistry, 2002 May 28;41(21):6805-16

Ø       "Expression of mammalian Rab Escort Protein -1 and -2 in yeast Saccharomyces cerevisiae". Vadim Sidorovitch, Anca Niculae, Natasha Kan, Alice-Corina Ceacareanu, Kirill Alexandrov. Protein expression and purification, (2002) 26 (1):50-58

 

2000

Ø       "Romanian population reveals six genetic clusters" - POSTER. Alice-Corina Ceacareanu, Augustin Ofiteru, Eleonora Luca, Corneliu Vulpe, Cristiana Glavce. National Congress of  Ecosystems Protection, Section of Biodiversity Bacau, Romania

Ø       "RAPD in human polymorphism: characteristics and limitations" - invited presentation. Alice-Corina Ceacareanu, Augustin Ofiteru, Bogdan Ceacareanu, Eleonora Luca, Corneliu Vulpe. National Congress of  Ecosystems Protection, Section of Biodiversity Bacau, Romania

Ø        "Confirming the presence of a very rare D1S80 allele in Romanian population" - invited presentation. Alice-Corina Ceacareanu, Mihaela Catana, Alexander Rodewald. National Congress of  Ecosystems Protection, Section of Biodiversity Bacau, Romania

Ø        "Distribution of Tsp 509 I subtype of D1S80 alleles in Romanian population"- Poster. Alice-Corina Ceacareanu, Augustin Ofiteru, Mihaela Catana, Alexander Rodewald. National Congress of  Ecosystems Protection, Section of Biodiversity Bacau, Romania

Ø       "Chemo-enzymatic synthesis of the fluorescent complex Rab7: GDP Dissociation Inhibitor" - POSTER. Alice-Corina Ceacareanu, Alexandra Hillebrand, Kirill Alexandrov. FEBS meeting, Spetses, Greece; XXIX National Congress of Immunology - National Society of Immunology Prize

 

1999

Ø       Parentage analysis for three Romanian families. Alice-Corina Ceacareanu, Bogdan Ceacareanu, Augustin Ofiteru, Geza Szegli. Rom. Arch. Imm. Microb. (1999), vol. 58, no. 2, 197-203) (English)

Ø       The relevance of paternity analysis in Romanian population using the D1S80 locus. Alice-Corina Ceacareanu, Bogdan Ceacareanu. Rom. Arch. Imm. Microb. (1999), vol. 58, no. 3-4, 281-289 (English)

 

1998

Ø       Molecular methods used in infectious agent taxonomy - poster. Gabriela Oprisan, Marietta Surdeanu, Alice-Corina Ceacareanu, Maria Damian, Dan Otelea. Medical Biology Reunion of Romanian Academy, Bucharest, Romania

Ø       Chimerical constructs of IL15 aimed to analyze the pathways of its production. Adrian Onu, Alice-Corina Ceacareanu, Geza Szegli. Rev. Roum. Biochim. (1998), 35, 1-2, 146-147

Ø       RAPD markers, indeed a possibility for human identification?Alice-Corina Ceacareanu, Bogdan Ceacareanu. Revue of National Society for Cellular Biology (June, 1999), no.27, 111-112

 

Citations

 

Biochemistry paper "Interaction of yeast Rab geranylgernyl transferase with its protein and lipid substrates" has been cited in:

Ø       The Rab6 GTPase Regulates Recruitment of the Dynactin Complex to Golgi Membranes; Benjamin Short, Christian Preisinger, Julia Schaletzky, Robert Kopajtich, and Francis A. Barr [Curr Biol 2002 Oct 15;12(20):1792-5]

Ø       Current awarness on yeasts: Published online in Wiley InterScience [Yeast 2002; I9: ll83-ll90]

Ø       Structure of Rab escort protein-1 in complex with Rab geranylgeranyltransferase; Pylypenko O, Rak A, Reents R, Niculae A, Sidorovitch V, Cioaca MD, Bessolitsyna E, Thoma NH, Waldmann H, Schlichting I, Goody RS, Alexandrov K [Mol Cell. 2003 Feb;11(2):483-94]

Ø       Handbook of Metalloproteins Vol. 3, (ISBN: 0-470-84984-3); Edited by Albrecht Messerschmidt, Wolfram Bode, Mirek Cygler;John Wiley & Sons, Ltd, Chichester, 2004 [Protein Prenyltransferases: pg 37-48]

Ø       Protein family review: Protein prenyltransferases; Maurer-Stroh S, Washietl S, Eisenhaber F [Genome Biology 2003 (4): 212.4-212.9]; http://genomebiology.com/2003/4/4/212

Ø       Semi-synthesis and Biological Evaluation of Prenylated Rab Proteins - PhD Dissertation thesis: Thomas Durek [Mak-Planck Institute for Molecular Physiology, Dortmund, Germany]

Ø       Structure of doubly prenylated Ypt1:GDI complex and the mechanism of GDI-mediated Rab recycling: Pylypenko O, Rak A, Durek T, Kushnir S, Dursina B, Thomae N, Constantinescu AT, Brunsweld L, Watzke A, Waldmann H, Goody RS, Alexandrov K [The EMBO Journal (2006) 25, 13-23]

 

Protein Expr. Purif. paper "Expression of mammalian Rab Escort Protein -1 and -2 in yeast Saccharomyces cerevisiae" has been cited in:

Ø       Structure of Rab escort protein-1 in complex with Rab geranylgeranyltransferase; Pylypenko O, Rak A, Reents R, Niculae A, Sidorovitch V, Cioaca MD, Bessolitsyna E, Thoma NH, Waldmann H, Schlichting I, Goody RS, Alexandrov K [Mol Cell. 2003 Feb;11(2):483-94]

Ø       Structure of the Rab7:REP-1 Complex:  Insights into the Mechanism of Rab Prenylation and Choroideremia Disease: Rak A, Pylypenko O, Niculae A, Pyatkov K, Goody RS, Alexandrov K [Cell 2004 (117): 749-760]

Ø       Synthesis of Fluorescently Labeled Mono- and Diprenylated Rab7 GTPase: Durek T, Alexandrov K, Goody RS, Hildebrand A, Heinemann I, Waldmann H [J. Am. Chem. Soc. 2004, 126, 16368-16378]

Ø       Course in Advanced Biochemistry and Molecular Biology BCMB8010 at the University of Georgia (www.arches.uga.edu/~kns/bcmb8010/smith.pdf )

Ø       Semi-synthesis and Biological Evaluation of Prenylated Rab Proteins - PhD Dissertation thesis: Thomas Durek [Mak-Planck Institute for Molecular Physiology, Dortmund, Germany]

Ø       Synthese und biologische Evaluierung semi-synthetischer Rab7-Proteine - PhD Dissertation thesis: Ines Heinemann [Mak-Planck Institute for Molecular Physiology, Dortmund, Germany]

 

Am J Physiol Heart Circ Physiol. paper "Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk" has been cited in:

Ø       Increase of PTP levels in vascular injury and in cultured aortic smooth muscle cells treated with specific growth factors: Chang Y, Zhuang D, Zhang C, Hassid A; [Am J Physiol Heart Circ Physiol. 2004 Nov;287(5):H2201-8]

Ø       Nitric oxide modulates microglial activation: Stefano GB, Kim E, Liu Y, Zhu W, Casaresc F, Mantione KJ, Jones D, Cadet P; [Med Sci Monit, 2004; 10(2): BR17-22]

Ø       Nitric oxide and cGMP protein kinase (cGK) regulate dendritic-cell migration toward the lymph-node-directing chemokine CCL19: Giordano D, Magaletti DM, Clark EA; [Blood, 2006(107): 1537-1545]

 

Am J Physiol Heart Circ Physiol paper "Nitric oxide attenuates insulin- or IGF-Istimulated aortic smooth muscle cell motility by decreasing H2O2 levels: essential role of cGMP" has been cited in:

Ø       Phagocytic NADPH oxidase overactivity underlies oxidative stress in metabolic syndrome: Fortuno A, San Jose G, Moreno MU, Beloqui O, zdiez J, Zalba G; [Diabetes, 2006(55): 200-215]

Ø       Nitric Oxide protects macrophages from hydrogen peroxide-induced apoptosis by inducing the formation of catalase: Yoshioka Y, Kitao K, Kishino T, Yamamuro A, Maeda S; [The Journal of Immunology, 2006, 176: 4675-4681]

Ø       Oklahoma Research Foundation, 2004 Scientific Report pg 78

 

Romanian Archives paper "Relevance of paternity analysis in Romanian population using D1S80 locus" has been cited in:

Ø       D1S80 VNTR locus genotypes in a population of Southeastern Sicily: distribution and genetic disequilibrium; Sciacca G, Grillo A, Paravizzini G, Barchitta M, Libra M, Emmanuele G, Callari D, Agodi A, Travali S [Am J Hum Biol; 16:91-94 (2004)]

Ø       Essential information and explanations, latest texts and monographs on paternity test; http://essential-facts/primary/biology-biologists/Paternity_test.html

 

Acknowledged Work

 

Ø       Journal of Managed Care Pharmacy, 2006, 12(7):570-577. Pharmacy benefit spending on oral chemotherapy drugs. Frederic R. Curtiss, RPh, PhD, Editor-in-Chief

Ø       Am J Physiol Heart Circ Physiol, 2004 (287):2201-2208. Increase of PTP levels in vascular injury and in cultured aortiec smooth muscle cells treated with specific growth factors. Chang Y, Zhuang D, Zhang C, Hassid A

Ø       Science, 24 Oct 2003, vol 302: 646- 650. Structure of Rab GDP-Dissociation Inhibitor in Complex with Prenylated YPT1 GTP-ase. Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, Waldmann H, Goody RS, Alexandrov K

 

Honors

 

2005-2007:        Pharm.D. Advanced Standing Student, admitted as a third year pharmacy student at University of Tennessee, College of Pharmacy, Memphis, US

2007:                Editorial Board Reviewer - Journal of Managed Care Pharmacy (JMCP)

2006:                Awarded with the AMCP / Pfizer Managed Care Internship for the project "Pharmacogenomic fingerprinting: perspectives in managed care"

2005:                Immigration and Naturalization Service granted US permanent residence based on outstanding research accomplishments

2002 - 2005:      Awarded with three years postdoctoral fellowship in cardiovascular diseases research at the University of Tennessee, Health Science Center, Memphis, Tennessee, United States

2001 - 2002:      Awarded with one year postdoctoral fellowship in pharmacology research at the University of Tennessee, Health Science Center, Memphis, Tennessee, United States

2000:                Awarded with the fundamental Immunology Prize "Ion Moraru" for the study "Chemo-enzimatic synthesis of the fluorescent complex RAB7:GDP dissociation inhibitor" at the 29th

                        Annual Meeting of the Romanian Society for Immunology (RSI), Bucharest, Romania. This award represents the highest recognition award of the RSI for young scientists performing their research in Health Care and Life Sciences. The opportunity for this award is less than 3%;

2000:                Awarded with the YTF Grant of Federation of European Biochemistry Societies (FEBS) for the advanced course "New dimensions in the regulation of gene expression", Spetses Island, Greece.

                        FEBS is the most recognized biochemistry society worldwide. Every year, the best young investigators in the field of biochemistry and molecular biology are selected and sponsored to receive advanced training in their area of research. The opportunity for this award is less than 10%.

1999:                Awarded with the YTF Grant from FEBS for the advanced course "Basic methods on yeast genetics and molecular biology", Louis Pasteur University, Strasbourg, France

1999:                Awarded with the Romanian Academy of Sciences Grant Award for the first study investigating human molecular polymorphism in Romania

                        This award is typically granted to very experienced investigators in the field of Health Care and Life Sciences. However, every 3-5 years young investigators may be considered for this award based on the national importance of their proposed research. The opportunity for young investigators is 1% or less

1991:                Ist prize, National Olympiad of Biology. Project: "Sephacryl HR2000 in the separation of proteins"

1990:                IIIr d prize, National Olympiad of Biology. Project: "Molecular mechanisms of our memory"

 

 

 

 

 

 

Domains of Competence

 

Clinical Pharmacy Training

 

Managing drug therapy and optimizing patient outcomes

 

Ø       Determine the presence of disease and medical condition

Ø       Determine the need for treatment or referral

Ø       Evaluate drug therapy for the presence of pharmacotherapeutic duplications and interactions

Ø       Identify contraindications, warnings, and precautions associated with a drug product's active and inactive ingredients

Ø       Identify physicochemical properties of drug substances that affect their solubility, pharmacokinetics, pharmacologic actions and stability

Ø       Interpret and apply pharmacokinetic principles to calculate and determine appropriate drug dosing regimens

Ø       Apply biopharmaceutical principles to assure bioavailability and enhance patient compliance

Ø       Determine the safety and effectiveness of pharmacotherapy

Ø       Identify and remedy adverse reactions and drug-induced illness

Ø       Prevent, recognize, and remedy noncompliance and drug misuse or abuse

 

Preparing medications and parenteral nutrition

Ø       Calculate the quantity of medication to be compounded or dispensed

Ø       Reduce or enlarge formulation quantities

Ø       Calculate the quantity or ingredients needed to compound the proper amount of the preparation

Ø       Calculate nutritional needs and the caloric content of nutrient sources

Ø       Calculate the rate of drug administration

Ø       Calculate or convert drug concentration

Ø       Identify the appropriate packaging, storage, handling, and disposal of medications

Ø       Interpret and apply pharmacokinetic parameters and quality assurance data to determine equivalence among manufactured drug products

Ø       Prepare and compound extemporaneous preparations and sterile products

Ø       Identify the important physicochemical properties of a preparation's active and inactive ingredients; describe the mechanism of, and the characteristic evidence of, incompatibility or degradation; and identify methods for achieving stabilization of the preparation

 

Providing drug information

Ø       Access, evaluate, and apply information to promote optimal health care

Ø       Interpret and evaluate data presented in textual, tabular or graphic form

Ø       Evaluate the suitability, accuracy, and reliability of information from reference sources by explaining and evaluating the adequacy of experimental design, and by applying and evaluating statistical tests and parameters

Ø       Educate patients and health care professionals regarding prescription medication, OTC, and medical services (provide information regarding medication's precautions, warnings, contraindications, and interactions with food, proper storage, administration and disposal)

Ø       Describe techniques for the self-monitoring of patients' health status

 

Advanced Laboratory Training

 

General biochemistry enzyme activity assays, protein separation, purification, quantification, concentration and crystallization; solubility tests and fluorescent labeling of in-vitro generated chimeras

 

Molecular biology / Molecular Genetics

Ø       Handling of bacterial cultures (experience with Top10, Top10F', DH5a, BL21, BL21DE3, BL21plysS, XLblue, Mycobacterium sp, Shigella sp, Streptococcus sp, enterotoxigenic E coli)

Ø       Handling of yeast cultures (Saccharomyces), including mitotic and meiotic mapping

Ø       Handling of adenoviruses for in vitro and in vivo delivery

Ø       DNA and RNA isolation and quantitation (total, nuclear, plasmidial, viral, cosmidic and phagemidic DNA; total and messenger RNA ); Sources: human, mammalians, plants, yeasts, bacteria, viruses

Ø       Gel-electrophoresis of nucleic acids (PAA, Agarosis, FIGE) and proteins

Ø       Polymerase Chain Reaction (PCR)

Ø       Randomized Amplified Polymorphism of DNA

Ø       Real Time - PCR

Ø       Reverse Transcribed - PCR

Ø       Restriction Fragment Length Polymorphism

Ø       Amplified Fragment Lenth Polymorphism

Ø       PCR mutagenesis, primer design, complementary DNA design, DNA cloning (plasmids and viruses), DNA/RNA sequencing

Ø       In vitro labeling (DNA - random and oligo-tailing)

Ø       DNA fingerprinting for epidemiology, plant identification and forensic investigation

Ø       Run-ON/Run-OFF nuclear assays for gene expression

Ø       Southern-blotting, Northern-blotting, Western-blotting

Ø       Fusion proteins design and expression (GST, Intein, Rluc, GFP, YFP)

 

Cellular Biology

Ø       Cell culture - CTLL, HeLa, Hep 2c, HEK293, COS 7

Ø       Primary cells isolation (rat and mouse): peritoneal lymphocytes, aortic smooth muscle cells

Ø       Obtaining of transient and stable transfected cell lines, Flow Cytometry

Ø       Signal transduction assays (receptor phosphorylation, protein phosphorylation, kinase assays, GTP-ase activity, cell migration)

Ø       Adenylyl cyclase assay on both living cells and isolated membranes

Ø       Ligand binding assays working on beta-adrenergic receptor

 

Microscopy

Ø       Inverted and Confocal microscopy

Ø       Phase contrast and fluorescence microscopy

 

Luminescence / Fluorescence Techniques

Ø       Investigation of physical molecular interaction by quantifying Bioluminescence Resonance Energy Transfer (BRET) in living cells

Ø       Quantifying free intracellular calcium in perfused living cells (Fura-2)

Ø       Quantifying Growth-factor induced H2O2 production in response to activation of signal transduction pathway activation in living aortic smooth muscle cells (DCF-DA labeling)

Ø       Bioengineering of fusion constructs containing green and yellow fluorescent proteins as well as Renilla luciferase (living cells)

 

Immunology Techniques

Ø       Animal handling (mouse, rat and rabbit)

Ø       Animal immunization for obtaining of hyper immune sera and polyclonal antibody purification

Ø       Enzyme Linked Immuno-Sorbent Assay

Ø       Immunohistochemistry - fluorescent staining, Haematoxillin / Eosin staining

Ø       Immunofluorescence, Immunoprecipitation, Immunoelectrophoresis

 

Toxicological Techniques

Ø       Toxicological expertise

Ø       Extraction and identification of barbiturates, benzodiazepines and other drugs in food, body liquids and environmental samples

Ø       Extraction and identification of inorganic toxic compounds

Ø       Identifying and quantifying barbiturates and benzodiazepines by Gas Chromatography, Gas Chromatography-Mass Spectrometry

 

Radioactivity Techniques

Ø       Experienced with radiolabeled compounds ( 3H, 32P, 125I)

 

 
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